[技術突破] 用噴灑的基因轉殖技術？ 20191107 BioRxiv

Plasmodesmata
Nov 7, 2019
2 min read

A simple method for spray-on gene editing in planta.

Cara Doyle, Katie Higginbottom, Thomas A. Swift, Mark Winfield, Christopher Bellas, David Benito-Alifonso, Taryn Fletcher, M. Carmen Galan, Keith Edwards, Heather M. Whitney

Abstract

Potential innovation in Plant research through the use of gene-edited and genetically modified plants is currently being hindered by inefficient and costly plant transformation. We show that naturally occurring carbon dots (quasi-spherical, <10nm nanoparticles) can act as a fast vehicle for carrying plasmids into mature plant cells, resulting in transient plant transformation in a number of important crop species with no negative impacts on photosynthesis or growth. We further show that GFP, Cas9, and gRNA introduced into wheat via foliar application (spraying on) of plasmid coated carbon dots are expressed and, in the case of Cas9, make genome edits in SPO11 genes. Therefore, we present a protocol for spray-on gene editing that is simple, inexpensive, fast, transforms in planta, and is applicable to multiple crop species. We believe this technique creates many opportunities for the future of plant transformation in research and shows great promise for plant protein production systems.

利用噴灑的方式來施行植物基因轉殖的簡單技術

摘要

在植物科學研究上，利用基因編輯以及基因修飾的方法來做有創新的潛力，正因為植物轉殖技術上的效率不高與成本高昂而限制住。我們展示了，自然生成的點狀碳（半球形，小於10奈米的奈米顆粒）可以當作一個高速的運載工具來運輸質體到成熟的植物細胞中，來短暫的轉殖數種不同的重要作物，並且不會影響光合作用的效率以及生長。我們更展示了利用噴灑帶有質體的點狀炭的方式，來將GFP、Cas9以及基因組DNA傳遞到小麥中，可以觀察到植體的表達，並且，在Cas9的例子中，我們可以將SPO11基因做基因編輯。因此我們呈現一個在植物中簡單、便宜、快速地噴灑式基因編輯實驗方法，而且這個方法是可以應用在多種作物上。我們相信這個新技術為未來在科學研究上的植物轉殖創造了許多新的契機，並且為植物蛋白質表達系統做了一個很好的展示。